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Understanding how proteins behave inside living cells is essential for modern drug discovery. Yet many widely used approaches, particularly plasmid overexpression systems and primary antibody–dependent assays, can alter or obscure the biology researchers aim to understand. Overexpression may push proteins above physiologically relevant levels, causing incorrect localization or artificial interactions. Antibody-driven detection strategies vary in… The post How Endogenous Tagging is Reshaping Protein Analysis appeared first on Drug Discovery and Development.
Work & Theory on January 1, 2026
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